pcdna3 egfp rhoa wt (Addgene inc)

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Addgene inc pcdna3 egfp rhoa wt
Pcdna3 Egfp Rhoa Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+egfp+rhoa+wt/pcDNA3-EGFP-RhoA-wt+(Plasmid+%2312965)/pm41392169-239-12-13
Average 93 stars, based on 43 article reviews

pcdna3 egfp rhoa wt - by Bioz Stars, 2026-09

93/100 stars

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Construct:

Article Title: RHOA regulates mitochondria-ER contact sites through modulation of the VAPB/PTPIP51 tether
Article Snippet: .. For the RHOA-GFP construct, RHOA-GFP was amplified by RHOA_OE_F and RHOA_OE_R from pcDNA3-EGFP-RHOA-wt (Addgene:12965) and assembled into AgeI/EcoRI digested pUltra backbone by Gibson assembly. ..

Article Title: RHOA regulates mitochondria-ER contact sites through modulation of the VAPB/PTPIP51 tether.
Article Snippet: .. For the RHOA-GFP construct, RHOA-GFP was amplified by RHOA_OE_F and RHOA_OE_R from pcDNA3-EGFP-RHOA-wt (Addgene:12965) and assembled into AgeI/EcoRI digested pUltra backbone by Gibson assembly. ..

Article Title: Myosin and \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upalpha$$\end{document} α -actinin regulation of stress fiber contractility under tensile stress
Article Snippet: Then, cells were transfected with a green fluorescent protein (GFP) constructs using the Nucleofector apparatus (Amaxa, Gaithersburg, MD) with Nucleofector kit VPI-1004 as previously described . .. The following fluorescent protein constructs were used: pcDNA3-EGFP-RhoA-wt (Addgene 12965) or vinculin-GFP (a gift from Kenneth Yamada, National Institute of Dental and Craniofacial Research, Bethesda, MD), and actin-mRFP (a gift from Michael Davidson, Florida State University, Tallahassee, FL). .. The microscope system used for these studies combines a CSU-22 Yokogawa spinning-disk confocal scanning head (Yokogawa Electric Inc, Japan) with a Bioscope SZ atomic force microscope (AFM) from Bruker Instruments Inc (Santa Barbara, CA) mounted on top of an inverted Olympus IX-81 optical microscope equipped with a PLAN APO 60 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\times$$\end{document} × oil 1.45 NA objective lens .

Amplification:

Over Expression:

Article Title: Single-cell metabolic imaging reveals a SLC2A3-dependent glycolytic burst in motile endothelial cells
Article Snippet: T7-eBFP2, T7-mCherry, and RhoA overexpression eBFP2 and mCherry constructs were generated from the Addgene plasmid #64323 and GeneCopoeia EX-C0726-M56-PFKFB3, respectively. .. For RhoA overexpression, pcDNA3-EGFP-RhoA-WT, pcDNA3-EGFP-RhoA-Q63L (Constituitively Active/CA), pcDNA3-EGFP-RhoA-T19N (Dominant Negative/DN) were purchased from Addgene (12965, 12968, 12967). .. EGFP was cloned out of each construct and replaced with either mScarlet-i (Addgene 85056 pLifeAct-mScarlet-i_N1) or eBFP2 using the vector and insert primers in Supplementary Table 1 , followed by Gibson assembly (NEB, E2611).

Plasmid Preparation:

Article Title: MIRO2 promotes cancer invasion and metastasis via MYO9B suppression of RhoA activity
Article Snippet: pCMV6-MIRO2-myc-FLAG was from Origene Technologies (catalog# RC204823). .. GFP-MYO9B (Addgene, plasmid# 134907) and GFP-MYO9B-R1695M (Addgene, plasmid# 134911) were a gift from Martin Bähler . pcDNA3-EGFP-RhoA-WT (Addgene, plasmid# 12965), pCDNA3-EGFP-RhoA-T19N (Addgene, plasmid# 12967), and pcDNA3-EGFP-RhoA-Q63L (Addgene, plasmid# 12968) were a gift from Gary Bokoch . dTomato-2xrGBD (Addgene plasmid# 129625) was a gift from Dorus Gadella . .. Empty vectors were matched to the backbone of the constructs, either pCMV6-entry (Origene Technologies) or pEGFP (Clontech).

Article Title: MIRO2 promotes cancer invasion and metastasis via MYO9B suppression of RhoA activity
Article Snippet: .. pcDNA3-EGFP-RhoA-WT , Subauste et al. , Addgene Plasmid #12965. .. pcDNA3-EGFP-RhoA-T19N , Subauste et al. , Addgene Plasmid #12967.

other:

Article Title: MIRO2 promotes cancer invasion and metastasis via MYO9B suppression of RhoA activity.
Article Snippet: Article

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